The role of DNA ligase in the construction of | vedclass

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(A) The primary role of $DNA$ ligase in the construction of a recombinant $DNA$ molecule is the formation of a phosphodiester bond between two $DNA$ f

Vedclass · Accepted Answer

Formation of phosphodiester bond between two $DNA$ fragments

Vedclass · Answer

(A) The primary role of $DNA$ ligase in the construction of a recombinant $DNA$ molecule is the formation of a phosphodiester bond between two $DNA$ fragments.$DNA$ ligase acts as a molecular glue by sealing the nicks or gaps in the sugar-phosphate backbone of $DNA$ strands.It catalyzes the joining of the $3'$-hydroxyl end of one nucleotide to the $5'$-phosphate end of another.This enzyme is essential for joining the vector $DNA$ and the insert $DNA$ to create a functional recombinant molecule.

The role of $DNA$ ligase in the construction of a recombinant $DNA$ molecule is:

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