The enzyme $DNA$ polymerase in $E. coli$ is a $DNA$-dependent polymerase and also has the ability to proof-read the $DNA$ strand being synthesized. Explain. Discuss the dual polymerase activity.

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(N/A) $DNA$ polymerase in $E. coli$ exhibits dual activity:
$1$. $DNA$-dependent $DNA$ polymerase activity: It catalyzes the polymerization of deoxyribonucleotides by reading the template $DNA$ strand,ensuring the synthesis of a complementary strand in the $5' \rightarrow 3'$ direction.
$2$. Proofreading activity ($3' \rightarrow 5'$ exonuclease activity): During replication,if an incorrect nucleotide is incorporated,the enzyme recognizes the mismatch. It then uses its $3' \rightarrow 5'$ exonuclease activity to remove the incorrect nucleotide and replaces it with the correct one,thereby ensuring high fidelity of replication.

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Similar Questions

Given below are the two statements.
Statement $I$: In prokaryotes,there is only one replicon,however,in eukaryotes,there are several replicons in tandem.
Statement $II$: The two separated strands in a replicon of $DNA$ are prevented from rejoining by helicase enzyme.
In the light of the above statements,choose the correct answer from the options given below.

$DNA$ replicates semiconservatively. This was experimentally proven by:

$DNA$ polymerase is required for:

Heavy $DNA$ is separated from normal $DNA$ based on which of the following?

Bacteria containing $^{15}N-DNA$ are grown in a medium containing $^{14}NH_4Cl$ for $80$ minutes. What will be the proportion of $DNA$?

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