The cutting of $DNA$ by restriction endonucleases results in the fragments of $DNA$. These fragments can be separated by a technique known as ......

The process of separation and purification of the expressed protein before marketing is called .........

What is meant by gene cloning?

Which of the following steps is $NOT$ used in the creation of Genetically Modified Organisms ($GMO$s)?

$A$ : Taq Polymerase is involved in $PCR$ technique.
$R$ : This enzyme remains active during the high temperature including denaturation of double stranded $DNA$.

Arrange the steps of $\text{rDNA}$ technology in the correct order$:-$
$(I.)$ Extraction of the desired gene product.
$(II.)$ Amplification of the gene of interest.
$(III.)$ Isolation of the desired $\text{DNA}$ fragment.
$(IV.)$ Ligation of the $\text{DNA}$ fragment into a vector.
$(V.)$ Insertion of $\text{rDNA}$ into the host.
The correct order is$:-$