Techniques that serve the purpose of early diagnosis include

  • A
    $PCR$
  • B
    $ELISA$
  • C
    $Serum\; analysis$
  • D
    $Both\; (a)\; and\; (b)$

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Statements related to human insulin are given below. Which statement$(s)$ is/are correct about genetically engineered insulin?
$(a)$ Pro-hormone insulin contains an extra stretch of $C$-peptide.
$(b)$ $A$-peptide and $B$-peptide chains of insulin were produced separately in $E. coli$,extracted,and combined by creating disulfide bonds between them.
$(c)$ Insulin used for treating diabetes was extracted from cattle and pigs.
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Identify the correct statement.

Gene therapy is an attempt to correct a genetic defect by providing a normal gene into the individual. By this,the normal function can be restored. An alternate method would be to provide the gene product (protein/enzyme) known as enzyme replacement therapy,which would also restore the function. Which in your opinion is a better option? Give reason for your answer.

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