Some of the steps of DNA fingerprinting are g | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(B) The correct sequence is $C-A-E-B-D$.The steps involved in $DNA$ fingerprinting are as follows:$1$. Isolation of $DNA$ from the sample cell.$2$. Am

Vedclass · Accepted Answer

$C-A-E-B-D$

Vedclass · Answer

(B) The correct sequence is $C-A-E-B-D$.The steps involved in $DNA$ fingerprinting are as follows:$1$. Isolation of $DNA$ from the sample cell.$2$. Amplification of $DNA$ (if required) using $PCR$.$3$. Digestion of $DNA$ by restriction endonucleases $(RENS)$ $(C)$.$4$. Separation of $DNA$ fragments by gel electrophoresis $(A)$.$5$. Transferring (blotting) of separated $DNA$ fragments to synthetic membranes like nitrocellulose or nylon $(E)$.$6$. Hybridisation using labelled variable number tandem repeat $(VNTR)$ probe $(B)$.$7$. Detection of hybridised $DNA$ fragments by autoradiography $(D)$.

Similar Questions

On what basis is satellite $DNA$ $NOT$ classified?

Read the following statements:
$A.$ Variation at genetic level arises due to mutations.
$B.$ The technique of $DNA$ fingerprinting was initially developed by Alec Jeffreys.

What is $DNA$ fingerprinting? Mention its application.

What is the correct sequence of steps in the process of $DNA$ fingerprinting?

Nowadays,it is possible to detect the mutated gene causing cancer by allowing a radioactive probe to hybridize with its complementary $DNA$ in a clone of cells,followed by its detection using autoradiography because:

Vedclass Test Series

Exam Paper Generator

Online Exam Module

Some of the steps of $DNA$ fingerprinting are given below. Identify the correct sequence from the options given.$A$. Electrophoresis of $DNA$ fragments$B$. Hybridisation with $DNA$ probe$C$. Digestion of $DNA$ by $RENS$$D$. Autoradiography$E$. Blotting of $DNA$ fragments to nitrocellulose membrane