Semi-conservative replication of DNA was firs | vedclass

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(A) The semi-conservative replication of $DNA$ was first experimentally demonstrated by Matthew Meselson and Franklin Stahl in $1958$.They used the ba

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Escherichia coli

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(A) The semi-conservative replication of $DNA$ was first experimentally demonstrated by Matthew Meselson and Franklin Stahl in $1958$.They used the bacterium $Escherichia$ $coli$ $(E. coli)$ for their experiments.They grew $E. coli$ in a medium containing $^{15}N$ (a heavy isotope of nitrogen) for many generations to label the $DNA$ with heavy nitrogen.Then,they transferred these bacteria to a medium containing $^{14}N$ (normal nitrogen) and extracted the $DNA$ at various time intervals.By analyzing the density of the $DNA$ using cesium chloride $(CsCl)$ density gradient centrifugation,they observed that the $DNA$ molecules were hybrids of both $^{15}N$ and $^{14}N$,which provided direct evidence for the semi-conservative mode of $DNA$ replication.

Column - $I$	Column - $II$
$(a)$ Helicase	$(1)$ Joining of nucleotides
$(b)$ Gyrase	$(2)$ Opening of $DNA$
$(c)$ Primase	$(3)$ Unwinding of $DNA$
$(d)$ $DNA$ Polymerase $III$	$(4)$ $RNA$ Priming

Semi-conservative replication of $DNA$ was first demonstrated in

Similar Questions

Identify the given model.

In which medium did Meselson and Stahl first culture $E. coli$?

The mode of $DNA$ replication is predominantly ......

Match the columns.
Column - $I$ Column - $II$
$(a)$ Helicase $(1)$ Joining of nucleotides
$(b)$ Gyrase $(2)$ Opening of $DNA$
$(c)$ Primase $(3)$ Unwinding of $DNA$
$(d)$ $DNA$ Polymerase $III$ $(4)$ $RNA$ Priming

In $DNA$ replication,in which direction does the synthesis of the new strand occur?

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