Semi-conservative replication of DNA was firs | vedclass

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(A) The semi-conservative replication of $DNA$ was first experimentally demonstrated by Matthew Meselson and Franklin Stahl in $1958$.They used the ba

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Escherichia coli

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(A) The semi-conservative replication of $DNA$ was first experimentally demonstrated by Matthew Meselson and Franklin Stahl in $1958$.They used the bacterium $Escherichia$ $coli$ $(E. coli)$ for their experiments.They grew $E. coli$ in a medium containing $^{15}N$ (a heavy isotope of nitrogen) for many generations to label the $DNA$ with heavy nitrogen.Then,they transferred these bacteria to a medium containing $^{14}N$ (normal nitrogen) and extracted the $DNA$ at various time intervals.By analyzing the density of the $DNA$ using cesium chloride $(CsCl)$ density gradient centrifugation,they observed that the $DNA$ molecules were hybrids of both $^{15}N$ and $^{14}N$,which provided direct evidence for the semi-conservative mode of $DNA$ replication.

Semi-conservative replication of $DNA$ was first demonstrated in

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