Polyethylene glycol method is used for

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(D) : Direct gene transfer is the transfer of naked $DNA$ into plant cells. Since the rigid plant cell wall acts as a barrier to $DNA$ uptake,protopla

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gene transfer without a vector

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(D) : Direct gene transfer is the transfer of naked $DNA$ into plant cells. Since the rigid plant cell wall acts as a barrier to $DNA$ uptake,protoplasts are the preferred target for this process. Polyethylene glycol $(PEG)$ mediated $DNA$ uptake is a direct gene transfer method that utilizes the interaction between $PEG$,naked $DNA$,salts,and the protoplast membrane to facilitate the transport of $DNA$ into the cytoplasm.

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