Modified antibiotics are manufactured by the technique of

Which of the following techniques gave birth to modern biotechnology?

What happens if there is no origin of replication $(ori)$ sequence in the $DNA$?

What is the fate of a piece of $DNA$ carrying only the gene of interest which is transferred into an alien organism?
$A$. The piece of $DNA$ would be able to multiply itself independently in the progeny cells of the organism.
$B$. It may get integrated into the genome of the recipient.
$C$. It may multiply and be inherited along with the host $DNA$.
$D$. The alien piece of $DNA$ is not an integral part of the chromosome.
$E$. It shows the ability to replicate.
Choose the correct answer from the options given below:

What is the full form of $EFB$?

Recombinant $DNA$ technology was discovered by