In the initial stages of protoplast culture, sorbitol/mannitol is added

  • A

    As an additional source of carbon

  • B

    As an additional source of carbon

  • C

    To keep cells alive after the removal of cell wall

  • D

    As a osmotic stabilizer

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  • [NEET 2014]

Pollen grains of a plant, whose $2 n=28$, are cultured to get callus by tissue culture method. What would be the number of chromosomes in the cells of the callus?