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The first clinical gene therapy was given in $1990$ to a $4$-year-old girl with an enzyme deficiency of:

How many recombinant therapeutics have been approved for human use in the world over?

Match the columns:
Column-$I$ Column-$II$
$(1)$ Diabetes $(a)$ $ADA$
$(2)$ Enzyme replacement therapy $(b)$ Alpha-lactalbumin
$(3)$ Emphysema $(c)$ Insulin
$(4)$ Rosie cow $(d)$ $\alpha-1$-Antitrypsin
$(e)$ Typhoid

$ELISA$ is used for the detection of viruses. The key reagent used is ......

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