Hardy-Weinberg equilibrium is not affected by which of the following factors?

Name the type of natural selection depicted in the given diagram (type $I,$ type $II$ and type $III$)

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Type $I$ Type $II$ Type $III$

 Definitions / Explanation: Gene Pool

Organic evolution would have not been taken place

Factor affecting the Hardy-Weinberg principles are

$I.$ gene flow

$II.$ genetic drift

$III.$ mutation

$IV.$ genetic recombination

$V.$ natural selection

The correct combination is

According to Hardy-Weinberg principle, allele and genotype frequencies in a population will remain constant from generation to generation in the absence of other evolutionary influences. It makes several assumptions which were given below.

$i.$  Random Mating

$ii.$  Sexual Reproduction

$iii.$  Non-overlapping Generations

$iv.$  Occurrence of Natural Selection

$v.$  Small size of population

Identify two assumptions which do not meet for a population to reach Hardy-Weinberg Equilibrium?