From the given combination of steps in PCR,id | vedclass

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(A) The Polymerase Chain Reaction $(PCR)$ consists of three main steps: Denaturation,Annealing,and Extension.$1$. Denaturation: The double-stranded $D

Vedclass · Accepted Answer

Extension only

Vedclass · Answer

(A) The Polymerase Chain Reaction $(PCR)$ consists of three main steps: Denaturation,Annealing,and Extension.$1$. Denaturation: The double-stranded $DNA$ is heated to high temperatures $(94-98^{\circ}C)$ to separate the strands. This is a physical process,not enzyme-dependent.$2$. Annealing: Primers bind to the single-stranded $DNA$ template at lower temperatures $(50-65^{\circ}C)$. This is also not enzyme-dependent.$3$. Extension: The enzyme $Taq$ $DNA$ polymerase adds nucleotides to the primer,extending the new $DNA$ strand. This step is strictly dependent on the activity of the $DNA$ polymerase enzyme.Therefore,the correct answer is Extension only.

From the given combination of steps in $PCR$,identify the enzyme-dependent step.

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