Class 11Chemistry8-3.Organic Chemistry : Purification and characterizationPurification of Organic Compounds
MediumExplain the principle of paper chromatography.
(N/A) Paper chromatography is a type of partition chromatography.
It involves the use of a special quality paper known as chromatography paper.
This paper contains water trapped in it,which acts as the stationary phase.
$A$ solution of the mixture is spotted on the base of this chromatography paper.
The paper strip is then suspended in a suitable solvent or a mixture of solvents,which acts as the mobile phase.
This solvent rises up the chromatography paper by capillary action and flows over the spot.
The components of the mixture are selectively retained on the paper based on their differing partition coefficients between the stationary phase (water) and the mobile phase.
As the mobile phase moves,the components travel to different heights on the paper.
The final paper strip obtained is known as a chromatogram.
It involves the use of a special quality paper known as chromatography paper.
This paper contains water trapped in it,which acts as the stationary phase.
$A$ solution of the mixture is spotted on the base of this chromatography paper.
The paper strip is then suspended in a suitable solvent or a mixture of solvents,which acts as the mobile phase.
This solvent rises up the chromatography paper by capillary action and flows over the spot.
The components of the mixture are selectively retained on the paper based on their differing partition coefficients between the stationary phase (water) and the mobile phase.
As the mobile phase moves,the components travel to different heights on the paper.
The final paper strip obtained is known as a chromatogram.
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