Exchange of germplasm is carried preferably through shoot tip culture because they are

Somaclonal variation appears in

Why is the culture continuously agitated in a rotary shaker during suspension culture?
$(i)$ To facilitate gas exchange for the cells.
$(ii)$ To ensure proper mixing of nutrients in the medium.
$(iii)$ To sterilize the equipment used in the process.
$(iv)$ To maintain a uniform temperature for the inoculum.
$(v)$ This process causes cell aggregates to break into smaller,distinct cell clusters.

$EDTA$ is widely used in tissue cultures; it is a:

Statement $P$: Callus culture medium contains auxin and cytokinin.
Statement $Q$: They are growth promoters and initiate cell division.

What is the required temperature for the induction of callus from an explant?