Enzymes have a very narrow optima for

As temperature changes from $3^o \,C$ to $45^o \,C$,the rate of enzyme activity will

Enzymes are proteins. Proteins are long chains of amino acids linked to each other by peptide bonds. Amino acids have many functional groups in their structure. Many of these functional groups are ionisable. As they are weak acids and bases in chemical nature,this ionisation is influenced by the $pH$ of the solution. For many enzymes,activity is influenced by the surrounding $pH$. This is depicted in the curve below. Explain briefly.

At which temperature do enzymes lose their native conformation?

At a temperature just below the freezing point,the enzyme:

According to the generally accepted rule,the rate of reaction doubles or halves for every $.........^{\circ}C$ change in temperature.