Discuss the significance of heavy isotope of nitrogen in the Meselson and Stahl’s experiment.

Vedclass pdf generator app on play store
Vedclass iOS app on app store

Matthew Meselson and Franklin Stahl performed the following experiment in $1958$ . They grew E. coli in a medium containing ${ }^{15} \mathrm{NH} 4 \mathrm{C} 1\left({ }^{15} \mathrm{~N}\right.$ is the heavy isotope of nitrogen) as the only nitrogen source for many generations. The result was that ${ }^{15} \mathrm{~N}$ was incorporated into newly synthesised $DNA$ (as well as other nitrogen containing compounds).

This heavy $DNA$ molecule could be distinguished from the normal DNA by centrifugation in a cesium chloride $(\mathrm{CsCl})$ density gradient ${ }^{15} \mathrm{~N}$ is not a radioactive isotope, and it can be separateDfrom ${ }^{14}  \mathrm{~N}$ only based on densities).

Similar Questions

In Meselson and Stahl's experiments, heavy $DNA$ was distinguished from normal $DNA$ by centrifugation in

Which one of the following correctly represents the manner of replication of $DNA$ ?

  • [AIIMS 2012]

$DNA$ replication is

Semiconservative model of $DNA$ replication was proposed by which workers in eukaryotes

Process of $DNA$ replication is effected by an enzyme called