Discuss the significance of heavy isotope of nitrogen in the Meselson and Stahl’s experiment.
Discuss the significance of heavy isotope of nitrogen in the Meselson and Stahl’s experiment.
Matthew Meselson and Franklin Stahl performed the following experiment in $1958$ . They grew E. coli in a medium containing ${ }^{15} \mathrm{NH} 4 \mathrm{C} 1\left({ }^{15} \mathrm{~N}\right.$ is the heavy isotope of nitrogen) as the only nitrogen source for many generations. The result was that ${ }^{15} \mathrm{~N}$ was incorporated into newly synthesised $DNA$ (as well as other nitrogen containing compounds).
This heavy $DNA$ molecule could be distinguished from the normal DNA by centrifugation in a cesium chloride $(\mathrm{CsCl})$ density gradient ${ }^{15} \mathrm{~N}$ is not a radioactive isotope, and it can be separateDfrom ${ }^{14} \mathrm{~N}$ only based on densities).
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