Definitions/Explanation : Probe & $VNTR$
 

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$(1)$ A labelled single stranded $DNA$ or $RNA$ segment that can hybridize with a $DNA$ or $RNA$ of interest in a screening procedure.

$(2)$The human genome contains numerous short $DNA$ sequences that are present as tandem repeats of varied lengths at several chromosomal locations. These variable number tandem repeats [$VNTRs$]are important components of $DNA$ fingerprints.

Similar Questions

Arrange the various steps of $DNA$ fingerprinting technique in the correct order

$(I)$ Separation of $DNA$ fragment by electrophoresis.

$(II)$ Digestion of $DNA$ by restriction endonucleases.

$(III)$ Hybridization using labeled $VNTR$ probe

$(IV)$ Isolation of $DNA$.

$(V)$ Detection of hybridized $DNA$ fragments by autoradiography.

$(VI)$ Transferring of separated $DNA$ fragment to synthetic membranes.

What is $DNA$ fingerprinting? Mention its application.

Write a explanatory note on $DNA$  Fingerprinting
 

$A$ : Unknown $DNA$ after hybridization with $VNTR$ probe, the autoradiogram gives many bands of differing sizes in $DNA$ profiling.
$R$ : These bands represents $DNA$ fingerprint of organism

$DNA$ fingerprinting involves identifying differences in some specific regions in $DNA$ sequence, called as:

  • [NEET 2021]