Bacterial colonies that have a foreign text{D | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(B) In the process of recombinant $	ext{DNA}$ technology,the $Lac \ Z$ gene encodes for the enzyme $\beta-$galactosidase. When a foreign $	ext{DNA}$

Vedclass · Accepted Answer

$\beta-$galactosidase gene shows insertional inactivation due to insertion of foreign $	ext{DNA}$

Vedclass · Answer

(B) In the process of recombinant $	ext{DNA}$ technology,the $Lac \ Z$ gene encodes for the enzyme $\beta-$galactosidase. When a foreign $	ext{DNA}$ fragment is inserted into this gene,it results in the disruption of the gene,a process known as insertional inactivation. As a result,the enzyme $\beta-$galactosidase is not produced. In the presence of a chromogenic substrate like $X-$gal,non-recombinant colonies (where the gene is intact) produce the enzyme,which cleaves $X-$gal to produce blue-colored colonies. Conversely,recombinant colonies (where the gene is inactivated) cannot produce the enzyme,so they cannot cleave $X-$gal and thus appear white.

Bacterial colonies that have a foreign $\text{DNA}$ fragment inserted into the plasmid will appear white,because $-$

Similar Questions

Stirred-tank bioreactors have been designed for

Which of the following methods are collectively referred to as downstream processing?

$PCR$ proceeds in three distinct steps governed by temperature. They are in order of:

Which steps are included in recombinant $DNA$ technology mechanism?

Vedclass Test Series

Exam Paper Generator

Online Exam Module