A student wishes to study the cell structure | vedclass

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(A) The resolution of a microscope is determined by the formula $d = \frac{0.61 \lambda}{NA}$,where $d$ is the limit of resolution,$\lambda$ is the wa

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(A) The resolution of a microscope is determined by the formula $d = \frac{0.61 \lambda}{NA}$,where $d$ is the limit of resolution,$\lambda$ is the wavelength of light used,and $NA$ is the numerical aperture of the objective lens.To achieve the best resolution,the value of $d$ should be as small as possible.Since $d$ is directly proportional to the wavelength $\lambda$,using light with the shortest wavelength will provide the highest resolution.Among the visible spectrum,blue light has the shortest wavelength (approximately $450-495 \ nm$) compared to green,yellow,or red light.Therefore,illuminating the object with blue light results in the best possible resolution.

$A$ student wishes to study the cell structure under a light microscope having $10X$ eyepiece and $45X$ objective. He should illuminate the object by which one of the following colours of light so as to get the best possible resolution?

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