$Escherichia$ $coli$ $(E. coli)$ is widely used in biological research because:

Assertion : In recombinant $DNA$ technology,human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason : Both bacteria and yeast multiply very fast to form huge population,which express the desired gene.

Match the following columns:

Column-$I$	Column-$II$
$(a)$ $EFB$	$(1)$ Recognition sequence
$(b)$ Biotechnology	$(2)$ Molecular scissors
$(c)$ Restriction enzyme	$(3)$ Definition of biotechnology
$(d)$ Palindromic sequence	$(4)$ Test tube baby

Assertion : $Agrobacterium$ $tumefaciens$ is popular in genetic engineering because this bacterium is associated with the roots of all cereal and pulse crops.
Reason : $A$ gene incorporated in the bacterial chromosomal genome gets automatically transferred to the crop with which the bacterium is associated.

Recombinant $DNA$ technology is related with

Find the correct match.

$I$	$II$	$III$
$(a)$ $PCR$	$(i)$ Specific base sequence	$p.$ $Taq$ polymerase
$(b)$ Downstream processing	$(ii)$ Inheritable gene	$q.$ Ampicillin resistant
$(c)$ Restriction endonuclease	$(iii)$ Amplification of $DNA$	$r.$ $E. coli$
	$(iv)$ Product separation and modification	$s.$ Quality control