Why is the culture continuously agitated in a rotary shaker during suspension culture?

In which method do cell clusters differentiate to form small,specialized cell groups?

Arrange the following steps of somatic hybridisation in a correct sequence.
$A$. Digestion of cell walls.
$B$. Isolation of naked protoplasts.
$C$. Fusion of protoplasts to get hybrid protoplast.
$D$. Isolation of single cells from two different varieties of plants.
$E$. Growing of hybrid protoplast to form a new plant.
Choose the correct answer from the options given below:

Scientists have succeeded in culturing meristems of:

Discuss how the property of plant cell totipotency has been utilised for plant propagation and improvement.

In order to obtain disease-free plants through tissue culture methods,the best technique is: