What are Gram-negative bacteria?

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(D) Gram staining is a technique used to differentiate bacterial species into two large groups: Gram-positive and Gram-negative.Gram-positive bacteria

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Bacteria that do not retain the Gram stain

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(D) Gram staining is a technique used to differentiate bacterial species into two large groups: Gram-positive and Gram-negative.Gram-positive bacteria have a thick peptidoglycan layer in their cell wall,which retains the crystal violet stain even after washing with alcohol,appearing purple.Gram-negative bacteria have a thinner peptidoglycan layer and an outer membrane,which causes them to lose the crystal violet stain during the decolorization process with alcohol.Therefore,Gram-negative bacteria are those that do not retain the Gram stain (crystal violet) and appear pink or red when counterstained with safranin.

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