Which of the following pairs is mismatched?

Choose the correct combination:

Column-$I$	Column-$II$
$A$. Escherichia coli	$I$. Nif gene
$B$. Rhizobium melilotae	$II$. Digestive hydrocarbon of crude oil
$C$. Bacillus thuringiensis	$III$. Production of human insulin
$D$. Pseudomonas putida	$IV$. Biological control of fungal disease
	$V$. Biodecomposed insecticide

Which of the following is essential to avoid environmental pollution?

Match the following columns:

Column-$I$	Column-$II$
$1.$ Silage	$a.$ Measure of water pollution level.
$2.$ Flocs	$b.$ Cattle feed.
$3.$ Biogas	$c.$ Bacteria associated with fungal filaments in water.
$4.$ $BOD$	$d.$ Anaerobic metabolism of organic waste.

Microbes are used in:
$1.$ Primary treatment of sewage
$2.$ Secondary treatment of sewage
$3.$ Anaerobic sludge digester
$4.$ Production of bioactive molecules

Which of the following statements are associated with silage?
$(i)$ Prepared with the help of $Propionibacterium$ $sharmanii$.
$(ii)$ Used in the preparation of bread.
$(iii)$ It is a type of cattle feed.
$(iv)$ Used for the production of ethanol.
$(v)$ Produced by the fermentation of carbohydrates present in green plant tissues.