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(D) Recombinant $DNA$ technology allows for the production of vaccines by inserting specific genes into host organisms.Both yeast (e.g.,$Saccharomyces

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Both $(A)$ and $(B)$

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(D) Recombinant $DNA$ technology allows for the production of vaccines by inserting specific genes into host organisms.Both yeast (e.g.,$Saccharomyces$ $cerevisiae$) and bacteria (e.g.,$Escherichia$ $coli$) are commonly used as host systems for the expression of recombinant proteins,including antigens used in vaccines.For example,the Hepatitis $B$ vaccine is produced using recombinant yeast cells.Therefore,both yeast and bacteria are utilized in this technology.

List-$I$	List-$II$
$A$. Genetically engineered Human Insulin	$I$. Gene therapy
$B$. $\text{GM}$ Cotton	$II$. $\text{E. coli}$
$C$. $\text{ADA}$ Deficiency	$III$. Antigen-antibody interaction
$D$. $\text{ELISA}$	$IV$. $\text{Bacillus thuringiensis}$

In which of the following microorganisms can vaccines be produced using recombinant $DNA$ technology?

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