In the Feulgen staining method,the Schiff rea | vedclass

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Question

(B) The Feulgen reaction is a staining technique used to identify chromosomal material or $DNA$ in cell nuclei. In this method,the tissue is subjected

Vedclass · Accepted Answer

The $CHO$ group of deoxyribose

Vedclass · Answer

(B) The Feulgen reaction is a staining technique used to identify chromosomal material or $DNA$ in cell nuclei. In this method,the tissue is subjected to mild acid hydrolysis (usually with $HCl$),which removes purine bases from the $DNA$. This process exposes the aldehyde group $(-CHO)$ of the deoxyribose sugar. The Schiff reagent then reacts with these exposed aldehyde groups to produce a magenta-colored compound,thereby staining the $DNA$.

In the Feulgen staining method,the Schiff reagent reacts with:

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