Which of the following restriction enzymes used in recombinant $DNA$ technology produces staggered cuts (sticky ends) in $DNA$?

Assertion : In recombinant $DNA$ technology,human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason : Both bacteria and yeast multiply very fast to form huge population,which express the desired gene.

Match List-$I$ with List-$II$:

List-$I$	List-$II$
$A$. Genetically modified organism	$I$. Agrobacterium tumefaciens
$B$. $DNA$ polymerase	$II$. Bt cotton
$C$. Ti plasmid	$III$. Thermus aquaticus
$D$. pBR322	$IV$. Escherichia coli

Choose the correct answer from the options given below:

$A$ - Transformation is a procedure through which a piece of $DNA$ is introduced in a host bacterium.
$R$ - With the help of $PCR$,thousands of $DNA$ copies are formed.

Identify the parts $A$ and $B$ from the given figure of the $pBR322$ cloning vector:

Match the following techniques or instruments with their usage:

Column-$I$	Column-$II$
$(a)$ Bioreactor	$(i)$ Separation of $DNA$ fragments
$(b)$ Electrophoresis	$(ii)$ Production of large quantities of products
$(c)$ $PCR$	$(iii)$ Detection of pathogen, based on antigen-antibody reaction
$(d)$ $ELISA$	$(iv)$ Amplification of nucleic acids

Select the correct option from the following: