A : DNA ligase plays an important role in rec | vedclass

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Question

(A) $DNA$ ligase is an enzyme that acts as a 'molecular glue' by catalyzing the formation of phosphodiester bonds between the $3'$-hydroxyl end of one

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Assertion and Reason both are correct and Reason is the correct explanation of Assertion.

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(A) $DNA$ ligase is an enzyme that acts as a 'molecular glue' by catalyzing the formation of phosphodiester bonds between the $3'$-hydroxyl end of one nucleotide and the $5'$-phosphate end of another.In recombinant $DNA$ technology,this enzyme is essential for joining the foreign $DNA$ fragment (gene of interest) with the cloning vector (such as a plasmid).The antibiotic resistance gene is a common marker used in plasmid vectors. To create a recombinant plasmid,the gene of interest and the vector are cut with the same restriction enzyme,and then $DNA$ ligase is used to covalently link them together.Therefore,both the Assertion and the Reason are correct,and the Reason provides a valid explanation for the importance of $DNA$ ligase in this technology.

$A$ : $DNA$ ligase plays an important role in recombinant $DNA$ technology.
$R$ : The linking of antibiotic resistance gene with plasmid vector became possible by enzyme $DNA$ ligase.

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$A$ : $DNA$ ligase plays an important role in recombinant $DNA$ technology.$R$ : The linking of antibiotic resistance gene with plasmid vector became possible by enzyme $DNA$ ligase.