Which enzyme is required to obtain DNA in its | vedclass

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Question

(A) To obtain $DNA$ in its pure form,the cell must be broken open to release $DNA$ along with other macromolecules such as $RNA$,proteins,polysacchari

Vedclass · Accepted Answer

Ribonuclease

Vedclass · Answer

(A) To obtain $DNA$ in its pure form,the cell must be broken open to release $DNA$ along with other macromolecules such as $RNA$,proteins,polysaccharides,and lipids.$RNA$ can be removed by treatment with ribonuclease $(RNase)$,and proteins can be removed by treatment with protease.Lysozyme is used to break the bacterial cell wall,while pectinase is used for plant cells.Restriction endonucleases are used to cut $DNA$ at specific sites,not for purification.Therefore,to ensure the $DNA$ is free from $RNA$ contamination,ribonuclease is the enzyme used.

Which enzyme is required to obtain $DNA$ in its pure form from a cell?

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