Describe the steps involved in $DNA$ fingerprinting.

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(N/A) $DNA$ fingerprinting involves identifying differences in specific regions in $DNA$ sequence called repetitive $DNA$. The steps are as follows:
$1$. Isolation of $DNA$.
$2$. Digestion of $DNA$ by restriction endonucleases.
$3$. Separation of $DNA$ fragments by electrophoresis.
$4$. Transferring (blotting) of separated $DNA$ fragments to synthetic membranes,such as nitrocellulose or nylon.
$5$. Hybridization using labelled $VNTR$ probe.
$6$. Detection of hybridized $DNA$ fragments by autoradiography.
$DNA$ fingerprinting was developed by Alec Jeffreys. It uses $VNTR$ (Variable Number of Tandem Repeats) as a probe because they show high degree of polymorphism.

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