Which of the following is not used in the Southern hybridization technique for chromosomal $DNA$ analysis?

  • A
    Electrophoresis
  • B
    Blotting
  • C
    Autoradiography
  • D
    $PCR$

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The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate.
Given below are two statements about this method :
Statement-$I$ : The blue coloured colonies have $\text{DNA}$ insert in the plasmid and they are identified as recombinant colonies.
Statement-$II$ : The colonies without blue colour have $\text{DNA}$ insert in the plasmid and are identified as recombinant colonies.
In the light of the above statements,choose the most appropriate answer from the options given below:

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