E. coli cells were allowed to replicate in a | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(B) $DNA$ replication is semi-conservative in nature.When $E. coli$ cells are grown in a medium containing radioactive thymidine,the newly synthesized

Vedclass · Accepted Answer

One strand will be radioactive.

Vedclass · Answer

(B) $DNA$ replication is semi-conservative in nature.When $E. coli$ cells are grown in a medium containing radioactive thymidine,the newly synthesized $DNA$ strands incorporate the radioactive label.After $5$ minutes,the $DNA$ molecules will have one radioactive strand (template) and one newly synthesized radioactive strand (if replication occurred) or just the labeled template.When these cells are transferred to a normal medium,the new strands synthesized will use non-radioactive nucleotides.Since the original $DNA$ molecule had one radioactive strand and one non-radioactive strand (or both radioactive depending on the duration),the resulting daughter $DNA$ molecules will retain the radioactive strand as a template.Specifically,in this experiment,one strand of the $DNA$ duplex will remain radioactive because it serves as the template for the new,non-radioactive strand.Therefore,one strand of the $DNA$ will be radioactive.

List-$I$	List-$II$
$a$. Ligase	Joins short segments of $\text{DNA}$ together.
$b$. $\text{DNA}$ polymerase	Cuts $\text{RNA}$ at specific $\text{RNA}$ sequence.
$c$. Helicase	Breaks the hydrogen bonds between complementary pairs during $\text{DNA}$ replication.

$E. coli$ cells were allowed to replicate in a medium containing radioactive thymidine for $5$ minutes and then transferred to a normal medium for further replication. Which of the following observations will be correct?

Similar Questions

On which of the following did Taylor and his colleagues perform their experiments?

$A$ discrete multiprotein structure on the chromosome which contains enzymes for $DNA$ duplication is called as

The process of $DNA$ replication is catalyzed by an enzyme called

Which enzyme is responsible for the synthesis of small $RNA$ fragments during $DNA$ replication?

Vedclass Test Series

Exam Paper Generator

Online Exam Module